Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Meridian Life Science Inhibitor-Tolerant RT-qPCR, 5ML
Meridian’s universal Inhibitor-Tolerant RT-qPCR Lo-ROX is a 4x formulation ready-to-use quantitative RT-PCR supermix, containing all components (including a passive reference dye) except primers, probes and templates for direct RT-qPCR. It is designed for developing RT-qPCR multiplex assays that require minimal sample processing and fast turn-around times (TAT). Because the mix is highly resistant to various RT and qPCR inhibitors, it can be used with crude lysates or inhibitor-rich samples to directly detect pathogens, including viruses and bacteria, in various biological specimens and to analyze gene expression in cells.
Cytek Biosciences Inc FastPROBE™ qPCR Lo-ROX Master Mix
FastPROBE™ qPCR Lo-ROX Master Mix ..... Cat. No. 31-5111-0500R ..... Size 500 rxns
INTACT GENOMICS INC ig SYBR Green qPCR 2X Master Mix 200 Reactions
Intact Genomics SYBR Green qPCR 2x master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA by qPCR. The Ig SYBR Green qPCR 2x master mix with integrated chemically modified hot start Taq DNA polymerase, SYBR Green I fluorescent dye, ROX dye*, MgCl2, dNTPs and stabilizers. This master mix is ideal for high-throughput real-time PCR screening and validation. This features a high-quality hot start Taq DNA Polymerase for higher fidelity and better amplification.Applications Gene expression validation Absolute quantification Mutation detection Pathogen detection GMO characterization Genetic profilingBenefits Enhanced efficiency, specificity, and sensitivity Compatible with all real-time PCR instruments Superior gene expression results under various cycling conditions Robust and active for cDNA synthesis at temperatures up to 55 °C.
Meridian Life Science Inhibitor-Tolerant qPCR Mix, 100ML
Meridian’s universal Inhibitor-Tolerant qPCR is a 2x formulation ready-to-use quantitative PCR supermix, containing all components except primers, probes and templates for direct qPCR. It is designed for developing qPCR multiplex assays that require minimal sample processing and fast turn-around times (TAT). Because the mix is highly resistant to various qPCR inhibitors, it can be used with crude lysates or inhibitor-rich samples to directly detect pathogens, including viruses and bacteria, in various biological specimens and to analyze gene expression in cells.
LUNA NANOTECH INC Micro Silica Spin Columns, 200 Columns
Have you ever ran out of columns that come with your favorite nucleic acid extraction kit but still had a lot of left-over buffer reagents remaining? Instead of buying a new expensive kit you can use PuroSPIN™ columns with the left-over buffers as a cost-effective alternative. Simply use PuroSPIN™ columns while following the manufacturer-specific protocols.PuroSPIN™ MICRO silica spin columns bind both DNA and RNALow 20 ?L elution volume - Maximizes concentration of purified product20 ?g nucleic acid binding capacityCompatible as cost-effective replacement columns for nucleic acid extraction kits from Qiagen, ThermoFisher, Bio-Rad, Promega and other manufacturersEach item includes both the spin column and a 2 mL sample collection tubeExtraction of DNA from bacteria, viruses, yeast, cultured cells, blood, urine, buccal swabs, animal tissue
LUNA NANOTECH INC Microplate Magnetic Separation Rack
96-well Microplate Magnetic Separation Rack
- Designed to be used with 96-well microplates, ELISA plates
- 96 magnets are positiont below microplate wells for efficient magnetic separation
- Can be used for high throughput separation of up to 96 samples
- Housing material: PMMA/Acrylic
- Magnet: N38 Neodymium (NdFeB)
Zymo Research Corporation Quick-cfRNA Binding Buffer, 100 ml
A component for Quick-cfRNA Serum & Plasma Kit
New England Biolabs, Inc. Luna Universal Probe qPCR Master Mix – 1000 rxn (10 x 1 ml)
The NEB Luna Universal Probe qPCR Master Mix is a 2X reaction mix optimized for real-time qPCR detection and quantitation of target DNA sequences using hydrolysis probes. It contains Hot Start Taq DNA Polymerase and has been formulated with a unique passive reference dye that is compatible across a variety of instrument platforms (including those that require a high or low ROX reference signal). It also features dUTP for carryover prevention and a non-fluorescent, visible dye to monitor reaction setup. This dye does not spectrally overlap fluorophores commonly used for qPCR and will not interfere with real-time detection. The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers/probes and DNA template. Genomic DNA or cDNA of interest can be quantitated with Luna qPCR and existing as well as commercial qPCR assay primer/probe sequences can be used.
NEW ENGLAND BIOGROUP LUNA 2nd General Reusable Slide, Counts 1 sample at a time, Contains LUNA reusable slide and one glass coverslip, For brightfield and fluorescent cell counts.
LUNA 2nd General Reusable Slide. Counts 1 sample at a time. Contains LUNA reusable slide and one glass coverslip. Can be used for brightfield and fluorescent cell counts. One year warranty
ACROBiosystems E1A resDNA Quantitation Kit (qPCR)
Residual host cell DNA refers to fragments of DNA from the host organism that are left behind after a biological process such as the production of a biopharmaceutical product or the cultivation of a cell line. This residual DNA can potentially contaminate the final product and affect its safety and efficacy. In the context of biopharmaceutical production, regulatory agencies such as the FDA and EMA have set limits on the amount of residual host cell DNA that is acceptable in a final product. These limits vary depending on the type of product and the route of administration, and residue host cell DNA quantitative kits are designed to ensure that the final product is safe for human use.
Roche Diagnostics KAPA PROBE FAST qPCR Kits - 500 x 20 uL reactions (ROX Low), 5 mL
Kits include reagents for RNA fragmentation, cDNA synthesis, and library preparation. Kits with reagents for mRNA capture are also available. *This item is non returnable.